Argonaute, a word of Greek origin, refers to a protein family that has a significant role in gene regulation and RNA interference. Synonyms for this word could include: RNA silencing, RNAi, gene silencing, gene regulation, small RNA, small interfering RNA, microRNA, gene expression, RNA-mediated gene silencing, and RNA-dependent gene silencing. Argonauts have a crucial role in regulating gene expression and development, suppressed virus infections, and provided information for therapeutic applications. From drug discovery to cancer therapy, Argonaute proteins have shown great potential to work as a biomarker or therapeutic target. Researchers and biologists use these synonyms as a reference to study RNA interference and gene regulation processes in the hope of finding a cure to some of the world's deadliest diseases.