Bacillus globigii, also known as Bacillus subtilis var. globigii, is a gram-positive, spore-forming bacterium commonly used as a biological indicator for process validation and sterilization procedures. This bacterium has also been used as a surrogate for other pathogenic bacteria due to its similar characteristics. Other synonyms for Bacillus globigii include Bacillus atrophaeus, Bacillus pumilus, and Bacillus licheniformis. These synonym strains have been used in various industrial sectors, such as food processing, pharmaceuticals, and agriculture, for their thermal resistance, biochemical activity, and sterilization characteristics. The use of Bacillus globigii and its synonyms plays a crucial role in maintaining the safety and efficacy of many industrial processes.