Cassette mutagenesis is a genetic engineering technique used to introduce targeted mutations into DNA. It involves the creation of a synthetic DNA fragment, known as a cassette, which is then inserted into the target DNA. However, there are several synonyms for cassette mutagenesis, including site-directed mutagenesis, oligonucleotide-mediated mutagenesis, and gene editing. Each of these techniques involves the targeted alteration of specific regions of DNA to introduce desired changes. Site-directed mutagenesis changes a single base pair at a specific location, whereas oligonucleotide-mediated mutagenesis introduces synthetic DNA fragments to cause mutations. Gene editing uses CRISPR/Cas technology to precisely edit DNA, making it a powerful tool for genome engineering.