You know, Sir, that queens generally receive the males about the fifth or sixth day, and this queen had not Copulated until the thirty-sixth.
"New observations on the natural history of bees"
Francis Huber
On the seventeenth we found no eggs in the hive; the queen was as slender as the first day; therefore the male, with which she had Copulated, had not impregnated her eggs.
"New observations on the natural history of bees"
Francis Huber