Fluorescein isothiocyanate (FITC) is a commonly used fluorescent label in biochemistry and molecular biology for labeling proteins, antibodies, and nucleic acids. FITC is known for its bright green fluorescence under excitation wavelengths between 465-490 nm. However, researchers may sometimes prefer to use other fluorescent labels depending on their specific experimental needs. Some popular alternatives to FITC include rhodamine, Cy3, and Alexa Fluor dyes. These labels offer different excitation and emission wavelengths, different photostability, and different signal intensities. By choosing the most suitable label for their experiment, researchers can ensure optimal sensitivity, specificity, and resolution in their fluorescence-based assays.