The antonyms of Gel Chromatography can be defined as alternative techniques or methods used for separating, purifying, and analyzing proteins and macromolecules. Some of the popular antonyms of Gel Chromatography include liquid chromatography, gas chromatography, affinity chromatography, ion-exchange chromatography, size-exclusion chromatography, and many more. Unlike Gel Chromatography, these alternative techniques offer a different mechanism for separating macromolecules based on their physicochemical properties such as charge, size, hydrophobicity, etc. Despite being an effective method, Gel Chromatography has limitations, such as the inability to separate high molecular weight compounds and sample loss during the filtration process. Therefore, researchers opt for different antonyms depending on their experimental requirements.