Massive parallel sequencing, also known as next-generation sequencing, is a high-throughput technology that enables the simultaneous sequencing of millions of DNA fragments in a single run. This technology has revolutionized the field of genomics as it allows for faster, more accurate and affordable analysis of genetic data. Synonyms for massive parallel sequencing include high-throughput sequencing, deep sequencing, and massively parallel sequencing. These terms are often used interchangeably and refer to the same technology. Other related terms include sequencing-by-synthesis, pyrosequencing, and single-molecule sequencing. Regardless of the name used, this technology has had a significant impact on our understanding of genetics and has led to advancements in personalized medicine, biomarker discovery, and disease diagnosis.