A Southwestern blot, also referred to as a DNA–protein hybridization assay, is a powerful technique used for detecting and studying DNA-protein interactions. Although the term "Southwestern blot" is widely recognized, it is helpful to explore some synonyms associated with this technique. Alternatives include DNA-protein band shift assay, DNA-protein interaction analysis, DNA-protein complex detection, or DNA-protein crosslinking analysis. These synonyms effectively convey the essence of the Southwestern blot method, which involves the hybridization of labeled DNA probes with target proteins to explore their interactions and investigate gene regulation at the protein level. By employing these diverse synonyms, researchers can communicate their findings effectively and broaden their knowledge in this field.