Transposon sequencing is a term used in genetics that refers to the study of the movement of genetic elements called transposons. This technique has helped scientists understand the regulation of gene expression, the identification of novel structural variants, and the identification of pathogenic mutations. There are several synonyms for this term, such as transposon insertion sequencing, transposon mutagenesis, and transposon-mediated DNA sequencing. These terms all describe the use of transposons to generate random mutations in a genome, followed by sequencing to identify the location and nature of the mutations. These techniques have proven to be powerful tools in understanding the genetic basis of diseases and in identifying new therapeutic targets.