The term sub-stratum refers to the layer of an underlying substance or foundation that is located beneath another layer. The opposite of sub-stratum is super-stratum, which refers to a layer located above another layer. Another antonym for sub-stratum is surface, which refers to the uppermost layer on top of the ground. The term apex is also an antonym for sub-stratum, as it refers to the highest point or peak of something. Lastly, the word pinnacle can be used as an antonym for sub-stratum, representing the highest point or climax of something.